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Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells - GSE87399 (Ensembl: Human Ensembl Gene)
Lack of allergy to timothy grass pollen is not a passive phenomenon but associated with allergen specific modulation of immune reactivity - GSE70050 (Ensembl: Human Ensembl Gene)
Genome-wide expression profiling of B Lymphocytes reveals IL4R increase in allergic asthma - GSE52742 (Ensembl: Human Ensembl Gene)
Gene Expression Patterns in Peripheral Blood Mononuclear Cells Associated with Asthma Exacerbation Attack - GSE19301 (Affymetrix: HG-U133A)
Gene expression patterns in house dust mite stimulated CD4 T cells and IgG to IgE ratios - GSE70760 (Affymetrix: HuGene-1_0-st_hugene10st_Hs_ENT)
Gene expression profiling of patients with allergy to latex and/or vegetable food - GSE13619 (Affymetrix: HG-Focus)
Integrated genomic and prospective clinical studies show the importance of modular pleiotropy for disease susceptibility, diagnosis and treatment (dataset 1) - GSE44956 (Agilent: SurePrint G3 Human GE Probe Name)
Gene expression changes in early phase of venom immunotherapy - GSE92866 (Affymetrix: Brainarray ENTREZG Version 20)
Distinct epithelial gene expression phenotypes in childhood respiratory allergy - GSE19190 - Disease State (Affymetrix: HG 1.0 ST)
Genome-wide expression analysis demonstrates a dominant role of TLR4 for activation of human phagocytes by the alarmin MRP8 - GSE56681 (Affymetrix: HG-U133_Plus_2)
Novel mediators of eicosanoid and epithelial nitric oxide production in asthma - GSE13785 (Affymetrix: HG-U133_Plus_2)
Functional classes of bronchial mucosa genes that are differentially expressed in asthma - GSE15823 (Affymetrix: HG_U95Av2)
Human basophil expression profiles - GSE64639 - Atopic vs Non-atopic (Illumina: HT12 V4)
The effect of a dexamethasone and a FK506 on the induction of chemokines in human mast cells - GSE15174new (Affymetrix: HG-U133_Plus_2)
Transcriptome profiling of 1) nasal polyp-derived human IL17RB positive and negative T-helper cells and 2) T-helper cells from normal nasal mucosa and matched peripheral blood - GSE70898_70900 (Illumina: HT12 V4)
Transcriptional profiling of egg allergy and relationship to disease phenotype - GSE88796 (Illumina: HT12 V4)
Neutrophil and PBMC gene expression data from Job's Syndrome - GSE8507 - PBMC (Affymetrix: HG-U133_Plus_2)
Neutrophil and PBMC gene expression data from Job's Syndrome - GSE8507 - Neutrophils (Affymetrix: HG-U133_Plus_2)
Leukotriene E4 is a full functional agonist for human cysteinyl leukotriene type 1 receptor - GSE75603 - Stimulation (Affymetrix: HG 1.0 ST)
Distinct epithelial gene expression phenotypes in childhood respiratory allergy - GSE19190 - Stimulation (Affymetrix: HG 1.0 ST)
Leukotriene E4 is a full functional agonist for human cysteinyl leukotriene type 1 receptor - GSE75603 - Cell Comparison (Affymetrix: HG 1.0 ST)
Gene expression profile of patients with moderate and severe chronic spontaneous urticaria - GSE72542 (Agilent: SurePrint G3)
Identification of IL-21-induced STAT3 dependent genes in human B cells - GSE51587 (Affymetrix: HG 1.0 ST)
Expression data from IBS patients before and after treatment - GSE14842 (Affymetrix: HG-U133_Plus_2)
Gene expression pattern of alveolar macrophages of allergic asthmatics in comparison with control subjects - GSE22528 (Affymetrix: HG-U133A)
Expression data for human epithelium from subjects with atopic dermatitis, psoriasis and nonatopic controls - GSE26952 (Illumina: Sentrix HuRef-8)
Progressive activation of Th2/Th22 characterizes acute and chronic atopic dermatitis - GSE36842 (Affymetrix: HG-U133_Plus_2)
Comparison of two sets of microarray experiments to define allergic asthma expression pattern - GSE41649 (Affymetrix: HG-U133A)
Gene expression analysis related to olive pollen allergy - GSE37157 (Affymetrix: HG-U133_Plus_2)
Influence of olive pollen stimuli on the gene- expression profile in healthy controls and allergic patients - GSE54522 (Affymetrix: HG-U133_Plus_2)
Upper airway gene expression is an effective surrogate biomarker for Th2-driven inflammation in the lower airway - GSE41861 (Affymetrix: HG-U133_Plus_2)
Effect of intradermal immunotherapy (IDIT) injections on gene expression profiles of activated T cells derived from skin biopsy explants - GSE72324 (Illumina: HT12 V4)
Illumina Bead expression array data from Human IgE+ and IgG+ B cell subsets - GSE99948 (Illumina: HT12 V4)
Gene Symbol: CCDC110
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Description

Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells - GSE87399

Purpose
Hypothesis
Allergen-specific immunotherapy has major effects on the frequency of peripheral Tfh and Tfr cells, which may be regulated by IL-2
Experimental Design
RNA-sequencing of sorted memory CXCR5 high and CXCR5low T cells from cryopreserved PBMC of 5 subjects with allergy and 5 patients undergoing allergen-specific immunotherapy
Experimental Variables

Allergy and allergen-specific immunotherapy.

Controls

5 subjects with allergy.

Methods

The single-end reads that passed Illumina filters were filtered for reads aligning to tRNA, rRNA, adapter sequences, and spike-in controls.RNA-Seq data was mapped against hg19 reference genome using tophat (v1.4.1., --library-type fr-secondstrand -C) and the RefSeq gene annotation downloaded from the UCSC genome bioinformatics site.The alignment results (sam files) were converted into bam files and sorted by using samtools.DUST scores were calculated with PRINSEQ Lite (v 0.20.3) and low-complexity reads (DUST > 4) were removed from the BAM files.Sequencing read coverage per gene was counted using HTSeq-count (-m union -s yes -t exon -i gene_id).Genome_build: hg19Supplementary_files_format_and_content: [.txt] file reports raw counts of sequencing reads per gene generated by HTSeq-count

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE87399

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BackgroundBiotinGenes001Genes005Gp95HousekeepingNoise
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