Transcriptional profiling of egg allergy and relationship to disease phenotype - GSE88796

To examine the transcriptional response to egg differed between PBMCs from egg allergic and clinically tolerant subjects.

PBMCs were obtained from egg allergic children, some of whom are baked egg responsive (BER, n = 34) and some are baked egg tolerant (BET, n = 20), as well as food allergic controls (AC, n = 14) who were clinically tolerant to egg. PBMCs were stimulated with egg antigen, gene transcription measured by microarray after 24 hours, and cytokine secretion by multiplex assay after 5 day incubation.

Egg antigen

14 non-allergic controls

PBMCs were isolated from whole blood by Ficoll-Paque PLUS, washed, and cultured in AimV with 2.5% autologous plasma. 4 x 106 cells were plated in 1 ml in 24-well culture plates, in the presence or absence of 300 ug of egg white (EW) protein cleaned of endotoxin using Detoxi-Gel columns.