Transcriptome profiling of 1) nasal polyp-derived human IL17RB positive and negative T-helper cells and 2) T-helper cells from normal nasal mucosa and matched peripheral blood - GSE70898_70900

To investigate the role of the IL-25/IL-33 axis in local mucosal T cell responses in CRSwNP.

TH2 cells characterize nasal polyps with a default TH17 signature in nasal

Polyp tissue and blood were obtained from patients undergoing nasal polypectomy. Control nasal biopsies and blood were obtained from healthy volunteers. Tissue was cultured in a short-term explant model. T cell surface phenotype/intracellular cytokines were assessed by flow cytometry. Cells were then activated with PMA/Ionomycin for 4hrs or left in a resting state.

PMA/Ionomycinm and expression of IL17RB

3 healthy donors

Polyp tissue and blood were obtained from patients undergoing nasal polypectomy. Control nasal biopsies and blood were obtained from healthy volunteers. Tissue was cultured in a short-term explant model. T cell surface phenotype/intracellular cytokines were assessed by flow cytometry. TCR V? analysis was performed with the immunoSEQ assay. Microarrays were performed for gene expression analysis.

The data correspond to the merge of GSE70898 and GSE70900 and further details can be found on the GEO website at:

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70898

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70900