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SIDRA

GXB

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Description

Genome-wide expression profiling of B Lymphocytes reveals IL4R increase in allergic asthma- GSE52742

Purpose

In this study we present the first genome-wide expression profiling of peripheral B cells by massive parallel RNA sequencing in patients with allergic asthma validating the discovery potential of this approach in allergy.

Experimental Design
RNA-seq was used to asses expression differences in B CD19 Lymphocytes from house dust mite allergic patients and healthy controls.
Experimental Variables

house dust mite allergy.

Controls

3 healthy controls.

Methods

Results were generated using the WASP system pipeline provided by Albert Einstein College of Medicine. Raw FASTQ files were trimmed for adapter sequences using quart. Alignment to the reference genome was performed using gsnap.Assignment of reads to genes was performed by htseq-count.Genome_build: HG 19Supplementary_files_format_and_content: A txt file format containing the Ensemble ID and the absolute number of counts per ID

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE52742

Platform Ensembl Human v37
No information available.
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(Uploaded through the Files tab in the Annotation Tool)

sampleset4000096_sampleannotations.csv

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