Sample Set Annotation Tool: Identification of IL-21-induced STAT3 dependent genes in human B cells

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

IL-21 induces B cell activation, and differentiation into antibody-secreting plasmablasts in vitro. This process is abolished by loss-of function mutations in STAT3. The objective of the study was to use microarrays to identify genes that are induced by IL-21 in a STAT3-dependent manner.

Hypothesis

Experimental Design

Sort-purified naïve B cells from 4 healthy donors and 3 patients with AD-HIES due to heterozygous STAT3 mutations were cultured for four days with CD40L in the presence or absence of IL-21 (50ng/ml). After four days, RNA was extracted from the harvested cells and the genetic profile was analysed by microarray.

Experimental Variables

STAT3 mutation, recombinant CD40 ligand and IL-21

Controls

4 healthy donors

Methods

B cells were stimulated with recombinant CD40 ligand alone or together with IL-21 (50 ng/mL).

Additional Information

Expected Samples:

14 Samples Loaded: 14

Species

Human (Homo sapiens)

Sample Source

B cells

Disease

Job's Syndrome (Hyper-IgE)

Treatment Protocol

B cells were stimulated with recombinant CD40 ligand alone or together with IL-21 (50 ng/mL)

Growth Protocol

Human naïve B cells were cultured in RPMI1640 containing 10% FCS for 4 days

Extraction Protocol

Trizol extraction of total RNA was performed according to the manufacturer's instructions.

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	diagnosis	Cell type	patient	Stimulation
GSM1248745	Healthy	naive B cells	donor 1	Healthy_CD40L
GSM1248746	Healthy	naive B cells	donor 2	Healthy_CD40L
GSM1248747	Healthy	naive B cells	donor 3	Healthy_CD40L
GSM1248748	Healthy	naive B cells	donor 4	Healthy_CD40L
GSM1248749	Healthy	naive B cells	donor 1	Healthy_CD40L_IL21

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File
GSE51587_v1.csv Sample Set Spreadsheet
GSE51587_v1.csv Sample Set Spreadsheet
GSE51587_v1.csv Sample Set Spreadsheet
GSE51587_v2.csv Sample Set Spreadsheet
GSE51587_v2.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	Diagnosis	Cell type	Patient	Stimulation
GSM1248745	Healthy	naive B cells	donor 1	Healthy_CD40L
GSM1248746	Healthy	naive B cells	donor 2	Healthy_CD40L
GSM1248747	Healthy	naive B cells	donor 3	Healthy_CD40L
GSM1248748	Healthy	naive B cells	donor 4	Healthy_CD40L
GSM1248749	Healthy	naive B cells	donor 1	Healthy_CD40L_IL21
GSM1248750	Healthy	naive B cells	donor 2	Healthy_CD40L_IL21
GSM1248751	Healthy	naive B cells	donor 3	Healthy_CD40L_IL21
GSM1248752	Healthy	naive B cells	donor 4	Healthy_CD40L_IL21
GSM1248753	AD-HIES	naive B cells	AD-HIES pt 1	ADHIES_CD40L
GSM1248754	AD-HIES	naive B cells	AD-HIES pt 2	ADHIES_CD40L
GSM1248755	AD-HIES	naive B cells	AD-HIES pt 3	ADHIES_CD40L
GSM1248756	AD-HIES	naive B cells	AD-HIES pt 1	ADHIES_CD40L_IL21
GSM1248757	AD-HIES	naive B cells	AD-HIES pt 2	ADHIES_CD40L_IL21
GSM1248758	AD-HIES	naive B cells	AD-HIES pt 3	ADHIES_CD40L_IL21

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