You are not logged in. | Log in
In Progress
Clinical

Distinct epithelial gene expression phenotypes in childhood respiratory allergy - GSE19190 - Stimulation

Purpose

In asthma, airway epithelium remodeling can already be detected during childhood, and epithelial cells are more susceptible to virus and oxidative stress. Their exact role in natural history and severity of children allergic respiratory disease remains however surprisingly unexplored. This study aimed tp analyze dysfunctions of epithelium in dust mite allergic respiratory disease (rhinitis ± asthma) in children.

Hypothesis

--

Experimental Design

Differentiated HNECs gene expression profiling in context of Th2 and IFN cytokine stimulation

Experimental Variables

IL4, IL13, IFNalpha, IFNbeta, and IFNgamma

Controls

Unstimulated controls

Methods

Expression profilings of nasal epithelial cells collected by brushing were performed on Affymetrix Hugene 1.0 ST arrays. All allergic patients were sensitized to dust mite. 19 patients had an isolated allergic rhinitis (AR). 14 patients had AR associated with asthma. Patients were compared to 12 controls, their severity and control being assessed according to NAEPP and ARIA criteria. Infections by respiratory viruses were excluded by real-time PCR measurements. Cells were treated with IL4, IL13, IFNalpha, IFNbeta, IFNgamma, or control for 24h.

Additional Information

--

Microarray
Affymetrix HuGene 1.0 ST v1
21 Samples Loaded: 21
Human (Homo sapiens)
Nasal epithelial tissue
Rhinitis
HNECs were treated with 10 ng/mL IL4 IL13 for 24 h or 1000 UI/mL IFNalpha, IFNbeta, or IFNgamma for 6 h
Absolutely RNA® Microprep Kit extraction of total RNA was performed according to the manufacturer's instructions.
Sample Set Spreadsheet
Please log in in order to upload files.
Samples Preview
Sample ID Cell type Stimulation Day Differentiated
GSM475535 HNECs IL4 D24
GSM475536 HNECs IL13 D24
GSM475537 HNECs Control D24
GSM475538 HNECs IL4 D25
GSM475539 HNECs IL13 D25
Please log in in order to upload files.
File
GSE19190 - Stimulation_v1.csv
Sample Set Spreadsheet
GSE19190 - Stimulation_v1.csv
Sample Set Spreadsheet
GSE19190 - Stimulation_v1.csv
Sample Set Spreadsheet
Log2 Transformed
Raw Signal
  •   |  View in GXB »
Clinical Datasource
Links

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID Cell type Stimulation Day Differentiated
GSM475535
HNECs
IL4
D24
GSM475536
HNECs
IL13
D24
GSM475537
HNECs
Control
D24
GSM475538
HNECs
IL4
D25
GSM475539
HNECs
IL13
D25
GSM475540
HNECs
Control
D25
GSM475541
HNECs
IL4
D26
GSM475542
HNECs
IL13
D26
GSM475543
HNECs
Control
D26
GSM475544
HNECs
IFNalpha
D21
GSM475545
HNECs
IFNbeta
D21
GSM475546
HNECs
IFNgamma
D21
GSM475547
HNECs
Control
D21
GSM475548
HNECs
IFNalpha
D24
GSM475549
HNECs
IFNbeta
D24
GSM475550
HNECs
IFNgamma
D24
GSM475551
HNECs
Control
D24
GSM475552
HNECs
IFNalpha
D25
GSM475553
HNECs
IFNbeta
D25
GSM475554
HNECs
IFNgamma
D25
GSM475555
HNECs
Control
D25

Group Sets View in Gene Expression Browser

Name  
All Samples   View
Stimulation Default  View
In order to make new Group Sets or change settings, please log in.

Module Analysis

Group Set  
All Samples
Stimulation
Your bug report has been sent!