Sample Set Annotation Tool: Leukotriene E4 is a full functional agonist for human cysteinyl leukotriene type 1 receptor - GSE75603

Study Design
Assay
Sample Info
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Analysis

Purpose

Leukotriene E4 (LTE4) the most stable of the cysteinyl leukotrienes (cysLTs) binds poorly to classical type 1 (CysLT1) and 2 (CysLT2) receptors although it induces potent responses in human airways in vivo, such as bronchoconstriction, airway hyperresponsiveness and inflammatory cell influx suggesting the presence of a novel receptor that preferentially responds to LTE4. The objective of the study was to to identify such a receptor, intracellular signalling and gene expression response to LTE4 were examined in two human mast cell lines, LAD2 and LUVA.

Hypothesis

Experimental Design

LAD2 cells were cultured and stimulated for 2 h with LTD4 (100nM), LTE4 (100nM) or medium (vehicle) control. LUVA cell were cultured. Total RNA was extracted and analysed using Affymetrix Human Gene 1.0 ST Arrays.

Experimental Variables

LTD4 and LTE4

Controls

Vehicle control

Methods

LAD2 cells were stimulated with either vehicle control, LTD4(100 nM) or LTE4(100nM) for 2 hours in the presence of L-cysteine (3 mmol/L).

Additional Information

Expected Samples:

9 Samples Loaded: 9

Species

Human (Homo sapiens)

Sample Source

Mast cell lines

Disease

Healthy

Treatment Protocol

LAD2 cells were stimulated with either vehicle control, LTD4(100 nM) or LTE4(100nM) for 2 hours in the presence of L-cysteine (3 mmol/L).

Growth Protocol

Extraction Protocol

Total cellular RNA was isolated using the miRNeasy mini kit (Qiagen) and DNAase treated (Ambion).

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	Stimulation	Cell line	Experiment
GSM1960284	LAD2 control_1	Control	LAD2	Stimulation
GSM1960285	LAD2 control_2	Control	LAD2	Stimulation
GSM1960286	LAD2 control_3	Control	LAD2	Stimulation
GSM1960287	LAD2 LTD4_1	LTD4	LAD2	Stimulation
GSM1960288	LAD2 LTD4_2	LTD4	LAD2	Stimulation

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File
GSE75603_v1.csv

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Sample ID	!Sample Title	Stimulation	Cell line	Experiment
GSM1960284	LAD2 control_1	Control	LAD2	Stimulation
GSM1960285	LAD2 control_2	Control	LAD2	Stimulation
GSM1960286	LAD2 control_3	Control	LAD2	Stimulation
GSM1960287	LAD2 LTD4_1	LTD4	LAD2	Stimulation
GSM1960288	LAD2 LTD4_2	LTD4	LAD2	Stimulation
GSM1960289	LAD2 LTD4_3	LTD4	LAD2	Stimulation
GSM1960290	LAD2 LTE4_1	LTE4	LAD2	Stimulation
GSM1960291	LAD2 LTE4_2	LTE4	LAD2	Stimulation
GSM1960292	LAD2 LTE4_3	LTE4	LAD2	Stimulation

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