Sample Set Annotation Tool: Neutrophil and PBMC gene expression data from Job's Syndrome - GSE8507

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Hyper IgE Recurrent Infection Syndrome (HIES or Job’s syndrome), is a rare disorder of immunity and connective tissue, typically manifest with boils, cyst-forming pneumonias, and extremely elevated serum IgE as well as retained primary dentition and bone abnormalities. Inheritance can be autosomal dominant. The aim of the study if to identify a specific defect or gene of interest.

Hypothesis

Experimental Design

Polymorphonuclear leukocytes (PMNs) and peripheral blood mononuclear cells (PBMCs) were isolated from whole blood from patients with Job's syndrome and healthy control subjects. PMNs and PBMCs were incubated with or without IgG- and C3bi-coated latex beads for 3 and 6 hours.

Experimental Variables

IgG- and C3bi-coated latex beads, time

Controls

10 healthy controls

Methods

PMNs and PBMCs were isolated from venous blood of each participant in accordance with a protocol approved by the Institutional Review Board for Human Subjects, National Institute of Allergy and Infectious Diseases.

Additional Information

Expected Samples:

90 Samples Loaded: 90

Species

Human (Homo sapiens)

Sample Source

Polymorphonuclear leukocytes

Disease

Job's Syndrome (Hyper-IgE)

Treatment Protocol

PMNs were incubated with or without IgG- and C3bi-coated latex beads for 3 and 6 hours.

Growth Protocol

Extraction Protocol

Total RNA was extracted with the Qiagen RNeasy kit according to the manufacturer's instructions.

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	group	Cell type	treatment	patient
GSM211011	Healthy_control_0	PMN	control	Patient1
GSM211012	Healthy_control_0	PMN	control	Patient2
GSM211013	Healthy_control_0	PMN	control	Patient3
GSM211014	Healthy_control_0	PMN	control	Patient4
GSM211015	Healthy_control_0	PMN	control	Patient5

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File
GSE8507_v2.csv Sample Set Spreadsheet
GSE8507_v3.csv Sample Set Spreadsheet
GSE8507_v3.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	Group	Time	Cell type	Treatment	Patient	Disease Status
GSM211011	Healthy_control_0	0 h	PMN	control	Patient1	Healthy
GSM211012	Healthy_control_0	0 h	PMN	control	Patient2	Healthy
GSM211013	Healthy_control_0	0 h	PMN	control	Patient3	Healthy
GSM211014	Healthy_control_0	0 h	PMN	control	Patient4	Healthy
GSM211015	Healthy_control_0	0 h	PMN	control	Patient5	Healthy
GSM211016	Healthy_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient1	Healthy
GSM211017	Healthy_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient2	Healthy
GSM211018	Healthy_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient3	Healthy
GSM211019	Healthy_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient4	Healthy
GSM211020	Healthy_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient5	Healthy
GSM211021	Healthy_control_3	3 h	PMN	control	Patient1	Healthy
GSM211022	Healthy_control_3	3 h	PMN	control	Patient2	Healthy
GSM211023	Healthy_control_3	3 h	PMN	control	Patient3	Healthy
GSM211024	Healthy_control_3	3 h	PMN	control	Patient4	Healthy
GSM211025	Healthy_control_3	3 h	PMN	control	Patient5	Healthy
GSM211026	Healthy_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient1	Healthy
GSM211027	Healthy_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient2	Healthy
GSM211028	Healthy_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient3	Healthy
GSM211029	Healthy_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient4	Healthy
GSM211030	Healthy_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient5	Healthy
GSM211031	Healthy_control_6	6 h	PMN	control	Patient1	Healthy
GSM211032	Healthy_control_6	6 h	PMN	control	Patient2	Healthy
GSM211033	Healthy_control_6	6 h	PMN	control	Patient3	Healthy
GSM211034	Healthy_control_6	6 h	PMN	control	Patient4	Healthy
GSM211035	Healthy_control_6	6 h	PMN	control	Patient5	Healthy
GSM211036	Healthy_control_0	0 h	PMN	control	Patient6	Healthy
GSM211037	Healthy_control_0	0 h	PMN	control	Patient7	Healthy
GSM211038	Healthy_control_0	0 h	PMN	control	Patient8	Healthy
GSM211039	Healthy_control_0	0 h	PMN	control	Patient9	Healthy
GSM211040	Healthy_control_0	0 h	PMN	control	Patient10	Healthy
GSM211041	Healthy_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient6	Healthy
GSM211042	Healthy_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient7	Healthy
GSM211043	Healthy_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient8	Healthy
GSM211044	Healthy_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient9	Healthy
GSM211045	Healthy_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient10	Healthy
GSM211046	Healthy_control_3	3 h	PMN	control	Patient6	Healthy
GSM211047	Healthy_control_3	3 h	PMN	control	Patient7	Healthy
GSM211048	Healthy_control_3	3 h	PMN	control	Patient8	Healthy
GSM211049	Healthy_control_3	3 h	PMN	control	Patient9	Healthy
GSM211050	Healthy_control_3	3 h	PMN	control	Patient10	Healthy
GSM211051	Healthy_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient6	Healthy
GSM211052	Healthy_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient7	Healthy
GSM211053	Healthy_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient8	Healthy
GSM211054	Healthy_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient9	Healthy
GSM211055	Healthy_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient10	Healthy
GSM211056	Healthy_control_6	6 h	PMN	control	Patient6	Healthy
GSM211057	Healthy_control_6	6 h	PMN	control	Patient7	Healthy
GSM211058	Healthy_control_6	6 h	PMN	control	Patient8	Healthy
GSM211059	Healthy_control_6	6 h	PMN	control	Patient9	Healthy
GSM211060	Healthy_control_6	6 h	PMN	control	Patient10	Healthy
GSM211061	HIES_control_0	0 h	PMN	control	Patient11	HIES
GSM211062	HIES_control_0	0 h	PMN	control	Patient12	HIES
GSM211063	HIES_control_0	0 h	PMN	control	Patient13	HIES
GSM211064	HIES_control_0	0 h	PMN	control	Patient14	HIES
GSM211065	HIES_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient11	HIES
GSM211066	HIES_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient12	HIES
GSM211067	HIES_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient13	HIES
GSM211068	HIES_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient14	HIES
GSM211069	HIES_control_3	3 h	PMN	control	Patient11	HIES
GSM211070	HIES_control_3	3 h	PMN	control	Patient12	HIES
GSM211071	HIES_control_3	3 h	PMN	control	Patient13	HIES
GSM211072	HIES_control_3	3 h	PMN	control	Patient14	HIES
GSM211073	HIES_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient11	HIES
GSM211074	HIES_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient12	HIES
GSM211075	HIES_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient13	HIES
GSM211076	HIES_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient14	HIES
GSM211077	HIES_control_6	6 h	PMN	control	Patient11	HIES
GSM211078	HIES_control_6	6 h	PMN	control	Patient12	HIES
GSM211079	HIES_control_6	6 h	PMN	control	Patient13	HIES
GSM211080	HIES_control_6	6 h	PMN	control	Patient14	HIES
GSM211081	HIES_control_0	0 h	PMN	control	Patient15	HIES
GSM211082	HIES_control_0	0 h	PMN	control	Patient16	HIES
GSM211083	HIES_control_0	0 h	PMN	control	Patient17	HIES
GSM211084	HIES_control_0	0 h	PMN	control	Patient18	HIES
GSM211085	HIES_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient15	HIES
GSM211086	HIES_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient16	HIES
GSM211087	HIES_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient17	HIES
GSM211088	HIES_IgG-C3bi LB_3	3 h	PMN	IgG-C3bi LB	Patient18	HIES
GSM211089	HIES_control_3	3 h	PMN	control	Patient15	HIES
GSM211090	HIES_control_3	3 h	PMN	control	Patient16	HIES
GSM211091	HIES_control_3	3 h	PMN	control	Patient17	HIES
GSM211092	HIES_control_3	3 h	PMN	control	Patient18	HIES
GSM211093	HIES_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient15	HIES
GSM211094	HIES_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient16	HIES
GSM211095	HIES_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient17	HIES
GSM211096	HIES_IgG-C3bi LB_6	6 h	PMN	IgG-C3bi LB	Patient18	HIES
GSM211097	HIES_control_6	6 h	PMN	control	Patient15	HIES
GSM211098	HIES_control_6	6 h	PMN	control	Patient16	HIES
GSM211099	HIES_control_6	6 h	PMN	control	Patient17	HIES
GSM211100	HIES_control_6	6 h	PMN	control	Patient18	HIES

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SIDRA