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Clinical

Human basophil expression profiles (in vitro) - GSE64639 Replace with superseries GSE64664

Purpose

Compare gene global gene expression changes upon anti-IgE stimulation in purified basophils of atopic and non-atopic subjects.

Hypothesis

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Experimental Design

All sampling from the 16 subjects was done in a 2 month period in November and December, i.e., outside the normal seasonal allergy exposure time frame for the Baltimore area. The category of non-atopics was based on self-reported histories of no known allergies. Atopic subjects were chosen solely on the basis of a history of allergies that included a previous diagnosis by a physician.

Experimental Variables

anti-IgE

Controls

10 Non-atopic donors

Methods

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Additional Information

This is a subject of the available dataset from GSE64639

Microarray
Illumina HumanHT-12 v4
16 Samples Loaded: 16
Human (Homo sapiens)
Peripheral blood
Healthy
mRNA was isolated by miRNEasy kit extractions and contained both mRNA and miRNA, RIN values > 6 (lower allowed because of presence of miRNA)
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample Title group Cell type
GSM1576058 Expt 25 - Releaser 1 Non-atopic basophils
GSM1576059 Expt 25 - Releaser 2 Non-atopic basophils
GSM1576060 Expt 25 - Non-releaser 1 Non-atopic basophils
GSM1576061 Expt 25 - Non-releaser 2 Non-atopic basophils
GSM1576062 Expt 26 - non-atopic 1 Non-atopic basophils
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File
GSE64639_v1.csv
Sample Set Spreadsheet
GSE64639_v1.csv
Sample Set Spreadsheet
Raw Signal
Raw Signal
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Clinical Datasource
Links

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID !Sample Title Group Cell type
GSM1576058
Expt 25 - Releaser 1
Non-atopic
basophils
GSM1576059
Expt 25 - Releaser 2
Non-atopic
basophils
GSM1576060
Expt 25 - Non-releaser 1
Non-atopic
basophils
GSM1576061
Expt 25 - Non-releaser 2
Non-atopic
basophils
GSM1576062
Expt 26 - non-atopic 1
Non-atopic
basophils
GSM1576063
Expt 26 - non-atopic 2
Non-atopic
basophils
GSM1576064
Expt 26 - non-atopic 3
Non-atopic
basophils
GSM1576065
Expt 26 - non-atopic 4
Non-atopic
basophils
GSM1576066
Expt 26 - non-atopic 5
Non-atopic
basophils
GSM1576067
Expt 26 - non-atopic 6
Non-atopic
basophils
GSM1576068
Expt 26 - atopic 1
Atopic
basophils
GSM1576069
Expt 26 - atopic 2
Atopic
basophils
GSM1576070
Expt 26 - atopic 3
Atopic
basophils
GSM1576071
Expt 26 - atopic 4
Atopic
basophils
GSM1576072
Expt 26 - atopic 5
Atopic
basophils
GSM1576073
Expt 26 - atopic 6
Atopic
basophils

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