Sample Set Annotation Tool: Gene expression changes in early phase of venom immunotherapy

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Genome-wide gene expression profiles of patients with severe hymenoptera venom allergy (HVA) were conducted to better understand the better the molecular processes underlying the acquisition of immune-tolerance during VIT (venom immunotherapy).

Hypothesis

Experimental Design

Study includes 11 HVA patients sampled prior VIT; 12 HVA patients sampled after 12months of VIT; 12 patients sampled between 1st and 2nd month of VIT; 12 individuals with negative skin tests for HVA; 12 beekeepers.

Experimental Variables

Venom immunotherapy

Controls

12 individuals with negative skin test

Methods

All patients undergone ultra-rush venom immunotherapy (VIT-UR) protocol. VIT-UR begins with the initial venom dose of 0.1 mcg and the summary dose of 111.1 mcg is reached within 3.5 hours. Maintenance dose of 100 mcg venom was administered every 4 weeks. Standardized lyophilized venom allergen extract (Pharmalgen Alk Abello) was used for build-up phase of VIT, and aluminum hydroxide adsorbed insect venom (Alutard-Alk Abello) for maintenance phase of therapy

Additional Information

Expected Samples:

59 Samples Loaded: 59

Species

Human (Homo sapiens)

Sample Source

Whole Blood

Disease

Allergy

Treatment Protocol

Growth Protocol

Extraction Protocol

All blood samples were drawn prior venom injections. 2.5ml of whole lood was collected on PAXgene Blood RNA Tubes. mRNA was isolated using PAXgene Blood miRNA Kit (QIAGEN) according to manufacturer manual.

Storage Conditions

Sample Set Spreadsheet

Please log in in order to upload files.

Samples Preview

Sample ID	Age	Gender	tissue	group	condition
GSM2438962	39	female	whole blood	HVA_prior	HVA
GSM2438963	39	female	whole blood	HVA_VIT	HVA
GSM2438964	47	male	whole blood	HVA_prior	HVA
GSM2438965	47	male	whole blood	HVA_VIT	HVA
GSM2438966	34	male	whole blood	HVA_prior	HVA

Please log in in order to upload files.

File
BSE92866_v1.csv Sample Set Spreadsheet
BSE92866_v1.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	Age	Gender	Tissue	Group	Condition	!ample Title	Blood count (%) [neutrophils / lymohocytes / monocytes / eosinophils / basophils]
GSM2438962	39	female	whole blood	HVA_prior	HVA	priorVIT_1	NA
GSM2438963	39	female	whole blood	HVA_VIT	HVA	after12monthsVIT_1	NA
GSM2438964	47	male	whole blood	HVA_prior	HVA	priorVIT_2	NA
GSM2438965	47	male	whole blood	HVA_VIT	HVA	after12monthsVIT_2	NA
GSM2438966	34	male	whole blood	HVA_prior	HVA	priorVIT_3	NA
GSM2438967	34	male	whole blood	HVA_VIT	HVA	after12monthsVIT_3	NA
GSM2438968	41	male	whole blood	HVA_prior	HVA	priorVIT_4	NA
GSM2438969	41	male	whole blood	HVA_VIT	HVA	after12monthsVIT_4	NA
GSM2438970	42	female	whole blood	HVA_VIT	HVA	after12monthsVIT_5	NA
GSM2438971	52	female	whole blood	HVA_prior	HVA	priorVIT_6	NA
GSM2438972	52	female	whole blood	HVA_VIT	HVA	after12monthsVIT_6	NA
GSM2438973	33	male	whole blood	Control	Control	control_7	NA
GSM2438974	27	male	whole blood	Control	Control	control_8	NA
GSM2438975	29	female	whole blood	Control	Control	control_9	NA
GSM2438976	26	female	whole blood	Control	Control	control_10	NA
GSM2438977	NA	female	whole blood	Control	Control	control_11	NA
GSM2438978	60	female	whole blood	Control	Control	control_12	NA
GSM2438979	64	female	whole blood	HVA_prior	HVA	priorVIT_13	NA
GSM2438980	64	female	whole blood	HVA_VIT	HVA	after12monthsVIT_13	NA
GSM2438981	47	female	whole blood	HVA_prior	HVA	priorVIT_14	NA
GSM2438982	47	female	whole blood	HVA_VIT	HVA	after12monthsVIT_14	NA
GSM2438983	63	male	whole blood	HVA_prior	HVA	priorVIT_15	NA
GSM2438984	63	male	whole blood	HVA_VIT	HVA	after12monthsVIT_15	NA
GSM2438985	73	male	whole blood	HVA_prior	HVA	priorVIT_16	NA
GSM2438986	73	male	whole blood	HVA_VIT	HVA	after12monthsVIT_16	NA
GSM2438987	57	female	whole blood	HVA_prior	HVA	priorVIT_17	NA
GSM2438988	57	female	whole blood	HVA_VIT	HVA	after12monthsVIT_17	NA
GSM2438989	39	male	whole blood	HVA_prior	HVA	priorVIT_18	NA
GSM2438990	39	male	whole blood	HVA_VIT	HVA	after12monthsVIT_18	NA
GSM2438991	18	male	whole blood	Control	Control	control_19	NA
GSM2438992	62	female	whole blood	Control	Control	control_20	NA
GSM2438993	28	female	whole blood	Control	Control	control_21	NA
GSM2438994	23	male	whole blood	Control	Control	control_22	NA
GSM2438995	22	male	whole blood	Control	Control	control_23	NA
GSM2438996	25	male	whole blood	Control	Control	control_24	NA
GSM2438997	16	male	whole blood	HVA_VIT	HVA	after1MonthVIT_25	62.2/28.4/7.3/1.5/0.6
GSM2438998	48	male	whole blood	HVA_VIT	HVA	after1MonthVIT_26	54.1/32.4/6.7/5.3/1.5
GSM2438999	40	female	whole blood	HVA_VIT	HVA	after1MonthVIT_27	51.4/36.5/9.4/2.1/0.6
GSM2439000	NA	female	whole blood	HVA_VIT	HVA	after1MonthVIT_28	63.3/27.5/7.6/1.2/0.4
GSM2439001	36	male	whole blood	HVA_VIT	HVA	after1MonthVIT_29	56.6/33.6/7.4/1.8/0.6
GSM2439002	89	male	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_30	69.7/21.8/7.2/0.3/1
GSM2439003	45	female	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_31	47.7/44.5/6.5/0.8/0.5
GSM2439004	21	female	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_32	46.7/38.8/9.6/3.9/1
GSM2439005	63	male	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_33	69.7/22.6/6.2/1/0.5
GSM2439006	83	male	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_34	53.9/33.4/9/2.7/1
GSM2439007	16	male	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_35	67.3/24.4/6.5/1/0.8
GSM2439008	62	male	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_36	51.1/35.1/10/3/0.8
GSM2439009	62	female	whole blood	HVA_VIT	HVA	after1MonthVIT_37	57.6/32.9/8.6/0.3/0.6
GSM2439010	19	female	whole blood	HVA_VIT	HVA	after1MonthVIT_38	54.7/31.4/9.6/2.9/1.1
GSM2439011	57	male	whole blood	HVA_VIT	HVA	after1MonthVIT_39	60.5/25.2/9.1/3.5/1.7
GSM2439012	64	female	whole blood	HVA_VIT	HVA	after1MonthVIT_40	46.6/43.8/6.2/2.6/0.8
GSM2439013	28	male	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_41	48.4/41.4/9.3/0/0.9
GSM2439014	72	male	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_42	64.2/23.7/10.7/1/0.4
GSM2439015	62	male	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_43	57.4/36.8/5/0.1/0.7
GSM2439016	37	female	whole blood	HVA_VIT	HVA	after1MonthVIT_44	66.3/23.8/7.1/2.1/0.7
GSM2439017	59	male	whole blood	HVA_VIT	HVA	after1MonthVIT_45	66.7/24.3/8.1/0.6/0.3
GSM2439018	32	female	whole blood	HVA_VIT	HVA	after1MonthVIT_46	55.2/31.6/8.7/3.7/0.8
GSM2439019	53	male	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_47	48.7/40.9/8.2/1.8/0.4
GSM2439020	71	male	whole blood	without signs of HVA_beekeeper	without signs of HVA	beekeeper_48	82.4/11.4/5/0.4/0.6

Group Sets View in Gene Expression Browser

Name
All Samples		View
Group	Default	View
In order to make new Group Sets or change settings, please log in.

Module Analysis

Group Set
All Samples
Group

SIDRA