Sample Set Annotation Tool: Influence of olive pollen stimuli on the gene- expression profile in healthy controls and allergic patients

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

The objective of the study was to use the microarray approach for studying the olive pollen response, taking advantage our previous results of T-cell epitope mapping on Ole e 1 molecule (the major allergen from olive pollen) in order to analyze the stimuli influence on the gene-expression of olive pollen allergic patients.

Hypothesis

Experimental Design

Peripheral blood mononuclear cells (PBMCs) from 6 healthy controls and 6 allergic subjects were stimulated 24 hours with olive pollen stimuli: Ole e 1 molecule and two Ole e 1 peptides previously defined as P2+3 (aa10-31), mainly recognized by non-allergic subjects (possible immunoregulatory epitope) and P10+12+13 (aa90-130), immunodominant T-cell epitope.

Experimental Variables

olive pollen extract (Olea), peptide 2+3 and peptide 10+12+13

Controls

6 healthy controls

Methods

PBMCs from 6 controls subjects and 6 Olive pollen–allergic patients (1,000,000 cls) were cultured with and without olive pollen stimuli (olive pollen extract (Olea), peptide 2+3 and peptide 10+12+13) during 24 hours.

Additional Information

Expected Samples:

46 Samples Loaded: 46

Species

Human (Homo sapiens)

Sample Source

PBMC

Disease

Healthy

Treatment Protocol

Growth Protocol

PBMCs isolated from peripheral blood by density gradient

Extraction Protocol

Total RNA was isolated from PBMCs (10^6 cells) using Trizol (Invitrogen, Carlsbad, CA, USA) extraction and isopropyl alcohol precipitation.

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	diagnosis	Cell type	treatment	Donor	Pollination Period
GSM1318200	healthy	PBMC	basal	C-1	POST
GSM1318201	healthy	PBMC	basal	C-2	POST
GSM1318202	healthy	PBMC	basal	C-3	POST
GSM1318203	healthy	PBMC	basal	C-1	PRE
GSM1318204	healthy	PBMC	basal	C-2	PRE

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File
GSE54522_v1.csv Sample Set Spreadsheet
GSE54522_v1.csv Sample Set Spreadsheet
GSE54522_v2.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	Diagnosis	Cell type	Treatment	Donor	Pollination Period	Groups	All Groups
GSM1318200	healthy	PBMC	basal	C-1	POST	healthy basal	healthy basal POST
GSM1318201	healthy	PBMC	basal	C-2	POST	healthy basal	healthy basal POST
GSM1318202	healthy	PBMC	basal	C-3	POST	healthy basal	healthy basal POST
GSM1318203	healthy	PBMC	basal	C-1	PRE	healthy basal	healthy basal PRE
GSM1318204	healthy	PBMC	basal	C-2	PRE	healthy basal	healthy basal PRE
GSM1318205	healthy	PBMC	basal	C-3	PRE	healthy basal	healthy basal PRE
GSM1318206	healthy	PBMC	olea	C-1	POST	healthy olea	healthy olea POST
GSM1318207	healthy	PBMC	olea	C-2	POST	healthy olea	healthy olea POST
GSM1318208	healthy	PBMC	olea	C-1	PRE	healthy olea	healthy olea PRE
GSM1318209	healthy	PBMC	olea	C-2	PRE	healthy olea	healthy olea PRE
GSM1318210	healthy	PBMC	olea	C-3	PRE	healthy olea	healthy olea PRE
GSM1318211	healthy	PBMC	P2+3	C-1	POST	healthy P2+3	healthy P2+3 POST
GSM1318212	healthy	PBMC	P2+3	C-2	POST	healthy P2+3	healthy P2+3 POST
GSM1318213	healthy	PBMC	P2+3	C-3	POST	healthy P2+3	healthy P2+3 POST
GSM1318214	healthy	PBMC	P2+3	C-1	PRE	healthy P2+3	healthy P2+3 PRE
GSM1318215	healthy	PBMC	P2+3	C-2	PRE	healthy P2+3	healthy P2+3 PRE
GSM1318216	healthy	PBMC	P2+3	C-3	PRE	healthy P2+3	healthy P2+3 PRE
GSM1318217	healthy	PBMC	P10+12+13	C-1	POST	healthy P10+12+13	healthy P10+12+13 POST
GSM1318218	healthy	PBMC	P10+12+13	C-2	POST	healthy P10+12+13	healthy P10+12+13 POST
GSM1318219	healthy	PBMC	P10+12+13	C-3	POST	healthy P10+12+13	healthy P10+12+13 POST
GSM1318220	healthy	PBMC	P10+12+13	C-1	PRE	healthy P10+12+13	healthy P10+12+13 PRE
GSM1318221	healthy	PBMC	P10+12+13	C-2	PRE	healthy P10+12+13	healthy P10+12+13 PRE
GSM1318222	healthy	PBMC	P10+12+13	C-3	PRE	healthy P10+12+13	healthy P10+12+13 PRE
GSM1318223	allergic	PBMC	basal	A-1	POST	allergic basal	allergic basal POST
GSM1318224	allergic	PBMC	basal	A-2	POST	allergic basal	allergic basal POST
GSM1318225	allergic	PBMC	basal	A-3	POST	allergic basal	allergic basal POST
GSM1318226	allergic	PBMC	basal	A-1	PRE	allergic basal	allergic basal PRE
GSM1318227	allergic	PBMC	basal	A-2	PRE	allergic basal	allergic basal PRE
GSM1318228	allergic	PBMC	basal	A-3	PRE	allergic basal	allergic basal PRE
GSM1318229	allergic	PBMC	olea	A-1	POST	allergic olea	allergic olea POST
GSM1318230	allergic	PBMC	olea	A-2	POST	allergic olea	allergic olea POST
GSM1318231	allergic	PBMC	olea	A-3	POST	allergic olea	allergic olea POST
GSM1318232	allergic	PBMC	olea	A-1	PRE	allergic olea	allergic olea PRE
GSM1318233	allergic	PBMC	olea	A-2	PRE	allergic olea	allergic olea PRE
GSM1318234	allergic	PBMC	olea	A-3	PRE	allergic olea	allergic olea PRE
GSM1318235	allergic	PBMC	P2+3	A-1	POST	allergic P2+3	allergic P2+3 POST
GSM1318236	allergic	PBMC	P2+3	A-2	POST	allergic P2+3	allergic P2+3 POST
GSM1318237	allergic	PBMC	P2+3	A-3	POST	allergic P2+3	allergic P2+3 POST
GSM1318238	allergic	PBMC	P2+3	A-1	PRE	allergic P2+3	allergic P2+3 PRE
GSM1318239	allergic	PBMC	P2+3	A-2	PRE	allergic P2+3	allergic P2+3 PRE
GSM1318240	allergic	PBMC	P2+3	A-3	PRE	allergic P2+3	allergic P2+3 PRE
GSM1318241	allergic	PBMC	P10+12+13	A-1	POST	allergic P10+12+13	allergic P10+12+13 POST
GSM1318242	allergic	PBMC	P10+12+13	A-2	POST	allergic P10+12+13	allergic P10+12+13 POST
GSM1318243	allergic	PBMC	P10+12+13	A-3	POST	allergic P10+12+13	allergic P10+12+13 POST
GSM1318244	allergic	PBMC	P10+12+13	A-1	PRE	allergic P10+12+13	allergic P10+12+13 PRE
GSM1318245	allergic	PBMC	P10+12+13	A-3	PRE	allergic P10+12+13	allergic P10+12+13 PRE

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SIDRA