Sample Set Annotation Tool: Expression data for human epithelium from subjects with atopic dermatitis, psoriasis and nonatopic controls

Study Design
Assay
Sample Info
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Analysis

Purpose

The expression and function of tight A1:R41 genes, in particular claudin-1, was studied in order to further understanding of the biology underlying atopic dermatitis.

Hypothesis

Experimental Design

Gene expression profiling was performed on total RNA from nonlesional epidermal samples from 5 subjects with atopic dermatitis, 4 subjects with psoriasis, and 5 nonatopic controls using the Illumina Sentrix HumanRef-8 Expression BeadChip.

Experimental Variables

Atopic dermatitis, Psoriasis

Controls

Nonlesional epidermal samples from 5 nonatopic controls

Methods

Additional Information

Expected Samples:

16 Samples Loaded: 16

Species

Human (Homo sapiens)

Sample Source

Skin

Disease

Atopic dermatitis

Treatment Protocol

Growth Protocol

Extraction Protocol

Total RNA was extracted from epidermis by using the QIAshredder spin column and RNeasy RNA isolation kits (Qiagen, Hilden, Germany).

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	Age	!Sample_title	Sex	race	rast (radioallergosorbent test)
GSM663510	20s	Nonlesional Epithelium Atopic Dermatitis rep1	m	Native American	positive
GSM663511	50s	Nonlesional Epithelium Atopic Dermatitis rep2	m	Caucasian	positive
GSM663512	20s	Nonlesional Epithelium Atopic Dermatitis rep3	f	African American	positive
GSM663513	40s	Nonlesional Epithelium Atopic Dermatitis rep4	f	Caucasian	positive
GSM663514	30s	Nonlesional Epithelium Atopic Dermatitis rep5	f	Caucasian	positive

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Sample ID	Age	!Sample Title	Sex	Race	Rast (radioallergosorbent test)	Ige (kiu/l)	Eosinophil level (cells/ml)	Ar or asthma	Easi/pasi	Disease group
GSM663510	20s	Nonlesional Epithelium Atopic Dermatitis rep1	m	Native American	positive	normal (0-48.5 kIU/L): 4136	normal (120-300 cells/ml): 990	YES, subject has history of AR (allergic rhinitis), asthma or both: YES	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 11.6	Atopic Dermatitis
GSM663511	50s	Nonlesional Epithelium Atopic Dermatitis rep2	m	Caucasian	positive	normal (0-48.5 kIU/L): 710	normal (120-300 cells/ml): 510	YES, subject has history of AR (allergic rhinitis), asthma or both: YES	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 34.6	Atopic Dermatitis
GSM663512	20s	Nonlesional Epithelium Atopic Dermatitis rep3	f	African American	positive	normal (0-48.5 kIU/L): 2578	normal (120-300 cells/ml): 290	YES, subject has history of AR (allergic rhinitis), asthma or both: YES	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 15.2	Atopic Dermatitis
GSM663513	40s	Nonlesional Epithelium Atopic Dermatitis rep4	f	Caucasian	positive	normal (0-48.5 kIU/L): 319	normal (120-300 cells/ml): 430	YES, subject has history of AR (allergic rhinitis), asthma or both: YES	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 11.3	Atopic Dermatitis
GSM663514	30s	Nonlesional Epithelium Atopic Dermatitis rep5	f	Caucasian	positive	normal (0-48.5 kIU/L): 36	normal (120-300 cells/ml): 100	YES, subject has history of AR (allergic rhinitis), asthma or both: YES	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 4.5	Atopic Dermatitis
GSM663515	30s	Nonlesional Epithelium Psoriasis rep1	m	African American	positive	normal (0-48.5 kIU/L): 224	normal (120-300 cells/ml): 360	YES, subject has history of AR (allergic rhinitis), asthma or both: no	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 34	Psoriasis
GSM663516	20s	Nonlesional Epithelium Psoriasis rep2	m	Caucasian	negative	normal (0-48.5 kIU/L): 31.2	normal (120-300 cells/ml): 110	YES, subject has history of AR (allergic rhinitis), asthma or both: no	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 3	Psoriasis
GSM663517	30s	Nonlesional Epithelium Psoriasis rep3	f	Caucasian	positive	normal (0-48.5 kIU/L): 49.3	normal (120-300 cells/ml): 10	YES, subject has history of AR (allergic rhinitis), asthma or both: YES	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 9.1	Psoriasis
GSM663518	30s	Nonlesional Epithelium Psoriasis rep4	f	Caucasian	negative	normal (0-48.5 kIU/L): 18.2	normal (120-300 cells/ml): 140	YES, subject has history of AR (allergic rhinitis), asthma or both: no	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 6.6	Psoriasis
GSM663519	20s	Nonlesional Epithelium Nonatopic Control rep1	m	Caucasian	negative	normal (0-48.5 kIU/L): 70.8	normal (120-300 cells/ml): 90	YES, subject has history of AR (allergic rhinitis), asthma or both: no	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 0	Nonatopic Control
GSM663520	20s	Nonlesional Epithelium Nonatopic Control rep2a	f	Caucasian	negative	normal (0-48.5 kIU/L): 2.5	normal (120-300 cells/ml): 50	YES, subject has history of AR (allergic rhinitis), asthma or both: YES	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 0	Nonatopic Control
GSM663521	20s	Nonlesional Epithelium Nonatopic Control rep2b	f	Caucasian	negative	normal (0-48.5 kIU/L): 2.5	normal (120-300 cells/ml): 50	YES, subject has history of AR (allergic rhinitis), asthma or both: YES	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 0	Nonatopic Control
GSM663522	40s	Nonlesional Epithelium Nonatopic Control rep3	m	Caucasian	negative	normal (0-48.5 kIU/L): 2	normal (120-300 cells/ml): 140	YES, subject has history of AR (allergic rhinitis), asthma or both: no	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 0	Nonatopic Control
GSM663523	40s	Nonlesional Epithelium Nonatopic Control rep4a	m	African American	negative	normal (0-48.5 kIU/L): 2.9	normal (120-300 cells/ml): 160	YES, subject has history of AR (allergic rhinitis), asthma or both: no	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 0	Nonatopic Control
GSM663524	40s	Nonlesional Epithelium Nonatopic Control rep4b	m	African American	negative	normal (0-48.5 kIU/L): 2.9	normal (120-300 cells/ml): 160	YES, subject has history of AR (allergic rhinitis), asthma or both: no	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 0	Nonatopic Control
GSM663525	20s	Nonlesional Epithelium Nonatopic Control rep5	m	Caucasian	positive	normal (0-48.5 kIU/L): 34.7	normal (120-300 cells/ml): 130	YES, subject has history of AR (allergic rhinitis), asthma or both: no	disease severity score, range (0-72). Arbitrarily, we assigned 0 to Controls. EASI is used for Atopic Dermatitis and PASI for Psoriasis: 0	Nonatopic Control

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