Sample Set Annotation Tool: Gene expression pattern of alveolar macrophages of allergic asthmatics in comparison with control subjects

Study Design
Assay
Sample Info
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Analysis

Purpose

The implication of alveolar macrophages (AM) in asthma, a Th2 disease, has not been well characterized. The purpose of this study was to better characterize alveolar macrophage phenotype of allergic asthmatic compared with normal subjects using genomic expression analyses.

Hypothesis

Experimental Design

Alveolar macrophage samples from 5 allergic asthmatic subjects and 5 from control subjects (without allergy and asthma) were subjected to chip analysis (Affymetrix Human Genome U133A Array).

Experimental Variables

Asthma

Controls

5 non-asthmatic controls

Methods

Gene expression of AM isolated from bronchoalveolar lavage of 5 allergic asthamtic and 5 healthy control subjects were compared.

Additional Information

Expected Samples:

10 Samples Loaded: 10

Species

Human (Homo sapiens)

Sample Source

Macrophages

Disease

Asthma

Treatment Protocol

Gene expression data from non allergic non asthmatic subject, without any stimulation.

Growth Protocol

Alveolar macrophages (AM) were purified by adherence (2h) from bronchoalveolar lavage (BAL)

Extraction Protocol

RNA was extracted using a silica gel-based Rneasy spin column protocol (Qiagen)

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	Cell type	Disease state
GSM559371	Alveolar macrophages from broncholaveolar lavage	normal
GSM559372	Alveolar macrophages from broncholaveolar lavage	normal
GSM559373	Alveolar macrophages from broncholaveolar lavage	normal
GSM559374	Alveolar macrophages from broncholaveolar lavage	normal
GSM559375	Alveolar macrophages from broncholaveolar lavage	normal

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File
GSE22528_v1.csv Sample Set Spreadsheet
GSE22528_v1.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	Cell type	Disease state
GSM559371	Alveolar macrophages from broncholaveolar lavage	normal
GSM559372	Alveolar macrophages from broncholaveolar lavage	normal
GSM559373	Alveolar macrophages from broncholaveolar lavage	normal
GSM559374	Alveolar macrophages from broncholaveolar lavage	normal
GSM559375	Alveolar macrophages from broncholaveolar lavage	normal
GSM559376	Alveolar macrophages from broncholaveolar lavage	asthma
GSM559377	Alveolar macrophages from broncholaveolar lavage	asthma
GSM559378	Alveolar macrophages from broncholaveolar lavage	asthma
GSM559379	Alveolar macrophages from broncholaveolar lavage	asthma
GSM559380	Alveolar macrophages from broncholaveolar lavage	asthma

Group Sets View in Gene Expression Browser

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Module Analysis

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SIDRA