Sample Set Annotation Tool: Neutrophil and PBMC gene expression data from Job's Syndrome - GSE8507

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Hyper IgE Recurrent Infection Syndrome (HIES or Job’s syndrome), is a rare disorder of immunity and connective tissue, typically manifest with boils, cyst-forming pneumonias, and extremely elevated serum IgE as well as retained primary dentition and bone abnormalities. Inheritance can be autosomal dominant. The aim of the study if to identify a specific defect or gene of interest.

Hypothesis

Experimental Design

Polymorphonuclear leukocytes (PMNs) and peripheral blood mononuclear cells (PBMCs) were isolated from whole blood from patients with Job's syndrome and healthy control subjects. PMNs and PBMCs were incubated with or without IgG- and C3bi-coated latex beads for 3 and 6 hours.

Experimental Variables

IgG- and C3bi-coated latex beads, time

Controls

5 healthy controls

Methods

PMNs and PBMCs were isolated from venous blood of each participant in accordance with a protocol approved by the Institutional Review Board for Human Subjects, National Institute of Allergy and Infectious Diseases.

Additional Information

Expected Samples:

51 Samples Loaded: 51

Species

Human (Homo sapiens)

Sample Source

PBMC

Disease

Job's Syndrome (Hyper-IgE)

Treatment Protocol

PBMCs were incubated with or without IgG- and C3bi-coated latex beads for 3 and 6 hours.

Growth Protocol

Extraction Protocol

Total RNA was extracted with the Qiagen RNeasy kit according to the manufacturer's instructions.

Storage Conditions

Sample Set Spreadsheet

Please log in in order to upload files.

Samples Preview

Sample ID	group	Cell type	treatment	Disease Status
GSM211101	Heathy_control_0	MO	control	Heathy
GSM211102	Heathy_control_0	MO	control	Heathy
GSM211103	Heathy_control_0	MO	control	Heathy
GSM211104	Heathy_control_0	MO	control	Heathy
GSM211105	Heathy_control_0	MO	control	Heathy

Please log in in order to upload files.

File
GSE8507_v1.csv
GSE8507_v3.1.csv Sample Set Spreadsheet
GSE8507_v3.1.csv Sample Set Spreadsheet
GSE8507_v3.1.csv Sample Set Spreadsheet
GSE8507_v3.1.csv Sample Set Spreadsheet
GSE8507_v3.csv Sample Set Spreadsheet
GSE8507_v3.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	Group	Time	Cell type	Treatment	Disease Status	Ptient
GSM211101	Heathy_control_0	0 h	MO	control	Heathy	Patient1
GSM211102	Heathy_control_0	0 h	MO	control	Heathy	Patient2
GSM211103	Heathy_control_0	0 h	MO	control	Heathy	Patient3
GSM211104	Heathy_control_0	0 h	MO	control	Heathy	Patient4
GSM211105	Heathy_control_0	0 h	MO	control	Heathy	Patient5
GSM211106	Heathy_control_0	0 h	MO	control	Heathy	Patient6
GSM211107	Heathy_control_0	0 h	MO	control	Heathy	Patient7
GSM211108	Heathy_control_0	0 h	MO	control	Heathy	Patient8
GSM211109	Heathy_control_0	0 h	MO	control	Heathy	Patient9
GSM211110	Heathy_control_0	0 h	MO	control	Heathy	Patient10
GSM211111	HIES_control_0	0 h	MO	control	HIES	Patient12
GSM211112	HIES_control_0	0 h	MO	control	HIES	Patient13
GSM211113	HIES_control_0	0 h	MO	control	HIES	Patient14
GSM211114	HIES_control_0	0 h	MO	control	HIES	Patient15
GSM211115	HIES_control_0	0 h	MO	control	HIES	Patient16
GSM211116	HIES_control_0	0 h	MO	control	HIES	Patient17
GSM211117	HIES_control_0	0 h	MO	control	HIES	Patient18
GSM211118	Heathy_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	Heathy	Patient1
GSM211119	Heathy_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	Heathy	Patient2
GSM211120	Heathy_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	Heathy	Patient3
GSM211121	Heathy_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	Heathy	Patient4
GSM211122	Heathy_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	Heathy	Patient5
GSM211123	Heathy_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	Heathy	Patient6
GSM211124	Heathy_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	Heathy	Patient7
GSM211125	Heathy_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	Heathy	Patient8
GSM211126	Heathy_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	Heathy	Patient9
GSM211127	Heathy_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	Heathy	Patient10
GSM211128	HIES_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	HIES	Patient12
GSM211129	HIES_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	HIES	Patient13
GSM211130	HIES_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	HIES	Patient14
GSM211131	HIES_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	HIES	Patient15
GSM211132	HIES_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	HIES	Patient16
GSM211133	HIES_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	HIES	Patient17
GSM211134	HIES_IgG-C3bi LB_3	3 h	MO	IgG-C3bi LB	HIES	Patient18
GSM211135	Healthy_control_3	3 h	MO	control	Healthy	Patient1
GSM211136	Healthy_control_3	3 h	MO	control	Healthy	Patient2
GSM211137	Healthy_control_3	3 h	MO	control	Healthy	Patient3
GSM211138	Healthy_control_3	3 h	MO	control	Healthy	Patient4
GSM211139	Healthy_control_3	3 h	MO	control	Healthy	Patient5
GSM211140	Healthy_control_3	3 h	MO	control	Healthy	Patient6
GSM211141	Healthy_control_3	3 h	MO	control	Healthy	Patient7
GSM211142	Healthy_control_3	3 h	MO	control	Healthy	Patient8
GSM211143	Healthy_control_3	3 h	MO	control	Healthy	Patient9
GSM211144	Healthy_control_3	3 h	MO	control	Healthy	Patient10
GSM211145	HIES_control_3	3 h	MO	control	HIES	Patient12
GSM211146	HIES_control_3	3 h	MO	control	HIES	Patient13
GSM211147	HIES_control_3	3 h	MO	control	HIES	Patient14
GSM211148	HIES_control_3	3 h	MO	control	HIES	Patient15
GSM211149	HIES_control_3	3 h	MO	control	HIES	Patient16
GSM211150	HIES_control_3	3 h	MO	control	HIES	Patient17
GSM211151	HIES_control_3	3 h	MO	control	HIES	Patient18

Group Sets View in Gene Expression Browser

Name
All Samples		View
Group	Default	View
In order to make new Group Sets or change settings, please log in.

Module Analysis

Group Set
All Samples
Group

SIDRA