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Clinical

The effect of a dexamethasone and a FK506 on the induction of chemokines in human mast cells - GSE15174

Purpose

The objective of this study was to comprehensively investigate FceRI-mediated chemokine induction in human mast cells and the effect of a corticosteroid (dexamethasone) and a calcineurin inhibitor (FK506).

Hypothesis

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Experimental Design

Human peripheral blood-derived mast cells were stimulated with anti-IgE antibody in the presence of dexamethasone or FK506. Gene expression profiles were evaluated using GeneChip Human Genome U133 plus 2.0 probe arrays (Affymetrix).

Experimental Variables

Stimulation with anti-IgE antibody in the presence of dexamethasone or FK506.

Controls

DMSO

Methods

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Additional Information

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Microarray
Affymetrix HG-U133_Plus_2
5 Samples Loaded: 5
Human (Homo sapiens)
PBMC , Mast cell lines
Healthy
Peripheral blood-derived mast cells agent: preincubated with vehicle control DMSO, FK506, or dexamethasone (DEX) and then stimulated with 15 ug/ml anti-IgE antibody or
Saito H et al., Nat Protoc. 2006;1(4):2178-83.
Total RNA was extracted with RNeasy (Qiagen) and digested with DNase I (Qiagen) according to the manufacturer's instructions.
Sample Set Spreadsheet
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Samples Preview
Sample ID agent Cell type
GSM378805 DMSO peripheral blood-derived mast cells
GSM378807 aIgE peripheral blood-derived mast cells
GSM378808 DEX_aIgE peripheral blood-derived mast cells
GSM378809 FK506_aIgE peripheral blood-derived mast cells
GSM378810 DEX_ FK506_aIgE peripheral blood-derived mast cells
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File
GSE15174_v1.csv
Sample Set Spreadsheet
GSE15174_v1.csv
Sample Set Spreadsheet
Average Normalized
Raw Signal
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Sample ID Agent Cell type
GSM378805
DMSO
peripheral blood-derived mast cells
GSM378807
aIgE
peripheral blood-derived mast cells
GSM378808
DEX_aIgE
peripheral blood-derived mast cells
GSM378809
FK506_aIgE
peripheral blood-derived mast cells
GSM378810
DEX_ FK506_aIgE
peripheral blood-derived mast cells

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