Distinct epithelial gene expression phenotypes in childhood respiratory allergy - GSE19190 - Disease State
To analyze dysfunctions of epithelium in dust mite allergic respiratory disease (rhinitis ± asthma) in children.
Airway epithelium remodeling plays a role in severity of children allergic respiratory diseases.
Nasal epithelium gene expression profiling of dust mite allergic children with isolated rhinitis, rhinitis associated with asthma and controls. 38 samples classified in 4 categories: 14 isolated rhinitis (R), 6 rhinitis with uncontrolled asthma (UA), 7 rhinitis with controlled asthma (CA) and 11 healthy subjects (C ).
Expression profilings of nasal epithelial cells collected by brushing were performed on Affymetrix Hugene 1.0 ST arrays. All allergic patients were sensitized to dust mite. 19 patients had an isolated allergic rhinitis (AR). 14 patients had AR associated with asthma. Patients were compared to 12 controls, their severity and control being assessed according to NAEPP and ARIA criteria. Infections by respiratory viruses were excluded by real-time PCR measurements.
Patients were compared to 12 controls, their severity and control being assessed according to NAEPP and ARIA criteria.
Nasal airway epithelial cells were brushed from children and immediately lysed with 100 µl of Absolutely RNA® Microprep Kit lysis buffer mixed with 0,7 µl of ?-Mercaptoethanol 14,2M then stored at -70°C. Absolutely RNA® Microprep Kit extraction of total RNA was performed according to the manufacturer's instructions.
Nasal epithelium gene expression profiling of dust mite allergic children with isolated rhinitis, rhinitis associated with asthma and controls. The data were analyzed with Expression Console using Affymetrix default analysis settings and Gene Level-RMA sketch as normalization method.
Sample ID | Age | Gender | !Sample_title | tissue | disease state |
---|---|---|---|---|---|
GSM475618 | 9.01 y | Male | Uncontrolled asthma UA1 | Nasal airway epithelium | Uncontrolled asthma |
GSM475619 | 14.46 y | Male | Rhinitis R4 | Nasal airway epithelium | Rhinitis |
GSM475620 | 15.96 y | Male | Controlled asthma CA1 | Nasal airway epithelium | Rhinitis with controlled asthma |
GSM475621 | 6.41 y | Male | Healthy C1 | Nasal airway epithelium | Healthy |
GSM475622 | 14.39 y | Female | Healthy C2 | Nasal airway epithelium | Healthy |
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Sample ID | Age | Gender | !Sample Title | Tissue | Disease state |
---|---|---|---|---|---|
GSM475618 | 9.01 y |
Male |
Uncontrolled asthma UA1 |
Nasal airway epithelium |
Uncontrolled asthma |
GSM475619 | 14.46 y |
Male |
Rhinitis R4 |
Nasal airway epithelium |
Rhinitis |
GSM475620 | 15.96 y |
Male |
Controlled asthma CA1 |
Nasal airway epithelium |
Rhinitis with controlled asthma |
GSM475621 | 6.41 y |
Male |
Healthy C1 |
Nasal airway epithelium |
Healthy |
GSM475622 | 14.39 y |
Female |
Healthy C2 |
Nasal airway epithelium |
Healthy |
GSM475623 | 14.16 y |
Male |
Healthy C3 |
Nasal airway epithelium |
Healthy |
GSM475624 | 7.81 y |
Male |
Healthy C4 |
Nasal airway epithelium |
Healthy |
GSM475625 | 8.82 y |
Female |
Uncontrolled asthma UA2 |
Nasal airway epithelium |
Uncontrolled asthma |
GSM475626 | 11.74 y |
Female |
Rhinitis R10 |
Nasal airway epithelium |
Rhinitis |
GSM475627 | 12.57 y |
Female |
Rhinitis R5 |
Nasal airway epithelium |
Rhinitis |
GSM475628 | 14.28 y |
Male |
Controlled asthma CA2 |
Nasal airway epithelium |
Rhinitis with controlled asthma |
GSM475629 | 6.73 y |
Female |
Controlled asthma CA3 |
Nasal airway epithelium |
Rhinitis with controlled asthma |
GSM475630 | 13.05 y |
Female |
Controlled asthma CA4 |
Nasal airway epithelium |
Rhinitis with controlled asthma |
GSM475631 | 14.38 y |
Male |
Healthy C5 |
Nasal airway epithelium |
Healthy |
GSM475632 | 16.11 y |
Male |
Rhinitis R12 |
Nasal airway epithelium |
Rhinitis |
GSM475633 | 12.12 y |
Male |
Healthy C6 |
Nasal airway epithelium |
Healthy |
GSM475634 | 8.62 y |
Female |
Rhinitis R1 |
Nasal airway epithelium |
Rhinitis |
GSM475635 | 7.05 y |
Male |
Rhinitis R2 |
Nasal airway epithelium |
Rhinitis |
GSM475636 | 13.91 y |
Male |
Rhinitis R6 |
Nasal airway epithelium |
Rhinitis |
GSM475637 | 8.71 y |
Female |
Rhinitis R11 |
Nasal airway epithelium |
Rhinitis |
GSM475638 | 7.50 y |
Female |
Healthy C11 |
Nasal airway epithelium |
Healthy |
GSM475639 | 9.81 y |
Male |
Uncontrolled asthma UA3 |
Nasal airway epithelium |
Uncontrolled asthma |
GSM475640 | 9.36 y |
Female |
Controlled asthma CA5 |
Nasal airway epithelium |
Rhinitis with controlled asthma |
GSM475641 | 11.32 y |
Female |
Controlled asthma CA6 |
Nasal airway epithelium |
Rhinitis with controlled asthma |
GSM475642 | 11.90 y |
Male |
Healthy C7 |
Nasal airway epithelium |
Healthy |
GSM475643 | 7.49 y |
Male |
Rhinitis R3 |
Nasal airway epithelium |
Rhinitis |
GSM475644 | 9.61 y |
Female |
Controlled asthma CA7 |
Nasal airway epithelium |
Rhinitis with controlled asthma |
GSM475645 | 14.00 y |
Female |
Healthy C8 |
Nasal airway epithelium |
Healthy |
GSM475646 | 9.32 y |
Female |
Rhinitis R7 |
Nasal airway epithelium |
Rhinitis |
GSM475647 | 9.637234771 y |
Male |
Uncontrolled asthma UA4 |
Nasal airway epithelium |
Uncontrolled asthma |
GSM475648 | 13.92744695 y |
Female |
Healthy C9 |
Nasal airway epithelium |
Healthy |
GSM475649 | 10.19575633 y |
Male |
Healthy C10 |
Nasal airway epithelium |
Healthy |
GSM475650 | 10.94592745 y |
Female |
Rhinitis R8 |
Nasal airway epithelium |
Rhinitis |
GSM475651 | 13.08692676 y |
Female |
Rhinitis R13 |
Nasal airway epithelium |
Rhinitis |
GSM475652 | 13.66461328 y |
Male |
Rhinitis R9 |
Nasal airway epithelium |
Rhinitis |
GSM475653 | 10.16563997 y |
Male |
Rhinitis R14 |
Nasal airway epithelium |
Rhinitis |
GSM475654 | 8.930869268 y |
Female |
Uncontrolled asthma UA5 |
Nasal airway epithelium |
Uncontrolled asthma |
GSM475655 | 8.093086927 y |
Male |
Uncontrolled asthma UA6 |
Nasal airway epithelium |
Uncontrolled asthma |
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