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Clinical

Distinct epithelial gene expression phenotypes in childhood respiratory allergy - GSE19190 - Disease State

Purpose

To analyze dysfunctions of epithelium in dust mite allergic respiratory disease (rhinitis ± asthma) in children.

Hypothesis

Airway epithelium remodeling plays a role in severity of children allergic respiratory diseases.

Experimental Design

Nasal epithelium gene expression profiling of dust mite allergic children with isolated rhinitis, rhinitis associated with asthma and controls. 38 samples classified in 4 categories: 14 isolated rhinitis (R), 6 rhinitis with uncontrolled asthma (UA), 7 rhinitis with controlled asthma (CA) and 11 healthy subjects (C ).

Experimental Variables

Expression profilings of nasal epithelial cells collected by brushing were performed on Affymetrix Hugene 1.0 ST arrays. All allergic patients were sensitized to dust mite. 19 patients had an isolated allergic rhinitis (AR). 14 patients had AR associated with asthma. Patients were compared to 12 controls, their severity and control being assessed according to NAEPP and ARIA criteria. Infections by respiratory viruses were excluded by real-time PCR measurements.

Controls

Patients were compared to 12 controls, their severity and control being assessed according to NAEPP and ARIA criteria.

Methods

Nasal airway epithelial cells were brushed from children and immediately lysed with 100 µl of Absolutely RNA® Microprep Kit lysis buffer mixed with 0,7 µl of ?-Mercaptoethanol 14,2M then stored at -70°C. Absolutely RNA® Microprep Kit extraction of total RNA was performed according to the manufacturer's instructions.

Additional Information

Nasal epithelium gene expression profiling of dust mite allergic children with isolated rhinitis, rhinitis associated with asthma and controls. The data were analyzed with Expression Console using Affymetrix default analysis settings and Gene Level-RMA sketch as normalization method.

Microarray
Affymetrix HuGene 1.0 ST v1
38 Samples Loaded: 38
Human (Homo sapiens)
Nasal epithelial tissue
Allergy
Nasal airway epithelial cells were brushed from children and immediately lysed with 100 µl of Absolutely RNA® Microprep Kit lysis buffer mixed with 0,7 µl of ?-Mercaptoethanol 14,2M then stored at -70°C.
Absolutely RNA® Microprep Kit extraction of total RNA was performed according to the manufacturer's instructions.
Sample Set Spreadsheet
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Samples Preview
Sample ID Age Gender !Sample_title tissue disease state
GSM475618 9.01 y Male Uncontrolled asthma UA1 Nasal airway epithelium Uncontrolled asthma
GSM475619 14.46 y Male Rhinitis R4 Nasal airway epithelium Rhinitis
GSM475620 15.96 y Male Controlled asthma CA1 Nasal airway epithelium Rhinitis with controlled asthma
GSM475621 6.41 y Male Healthy C1 Nasal airway epithelium Healthy
GSM475622 14.39 y Female Healthy C2 Nasal airway epithelium Healthy
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Log2 Transformed
Raw Signal
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All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID Age Gender !Sample Title Tissue Disease state
GSM475618
9.01 y
Male
Uncontrolled asthma UA1
Nasal airway epithelium
Uncontrolled asthma
GSM475619
14.46 y
Male
Rhinitis R4
Nasal airway epithelium
Rhinitis
GSM475620
15.96 y
Male
Controlled asthma CA1
Nasal airway epithelium
Rhinitis with controlled asthma
GSM475621
6.41 y
Male
Healthy C1
Nasal airway epithelium
Healthy
GSM475622
14.39 y
Female
Healthy C2
Nasal airway epithelium
Healthy
GSM475623
14.16 y
Male
Healthy C3
Nasal airway epithelium
Healthy
GSM475624
7.81 y
Male
Healthy C4
Nasal airway epithelium
Healthy
GSM475625
8.82 y
Female
Uncontrolled asthma UA2
Nasal airway epithelium
Uncontrolled asthma
GSM475626
11.74 y
Female
Rhinitis R10
Nasal airway epithelium
Rhinitis
GSM475627
12.57 y
Female
Rhinitis R5
Nasal airway epithelium
Rhinitis
GSM475628
14.28 y
Male
Controlled asthma CA2
Nasal airway epithelium
Rhinitis with controlled asthma
GSM475629
6.73 y
Female
Controlled asthma CA3
Nasal airway epithelium
Rhinitis with controlled asthma
GSM475630
13.05 y
Female
Controlled asthma CA4
Nasal airway epithelium
Rhinitis with controlled asthma
GSM475631
14.38 y
Male
Healthy C5
Nasal airway epithelium
Healthy
GSM475632
16.11 y
Male
Rhinitis R12
Nasal airway epithelium
Rhinitis
GSM475633
12.12 y
Male
Healthy C6
Nasal airway epithelium
Healthy
GSM475634
8.62 y
Female
Rhinitis R1
Nasal airway epithelium
Rhinitis
GSM475635
7.05 y
Male
Rhinitis R2
Nasal airway epithelium
Rhinitis
GSM475636
13.91 y
Male
Rhinitis R6
Nasal airway epithelium
Rhinitis
GSM475637
8.71 y
Female
Rhinitis R11
Nasal airway epithelium
Rhinitis
GSM475638
7.50 y
Female
Healthy C11
Nasal airway epithelium
Healthy
GSM475639
9.81 y
Male
Uncontrolled asthma UA3
Nasal airway epithelium
Uncontrolled asthma
GSM475640
9.36 y
Female
Controlled asthma CA5
Nasal airway epithelium
Rhinitis with controlled asthma
GSM475641
11.32 y
Female
Controlled asthma CA6
Nasal airway epithelium
Rhinitis with controlled asthma
GSM475642
11.90 y
Male
Healthy C7
Nasal airway epithelium
Healthy
GSM475643
7.49 y
Male
Rhinitis R3
Nasal airway epithelium
Rhinitis
GSM475644
9.61 y
Female
Controlled asthma CA7
Nasal airway epithelium
Rhinitis with controlled asthma
GSM475645
14.00 y
Female
Healthy C8
Nasal airway epithelium
Healthy
GSM475646
9.32 y
Female
Rhinitis R7
Nasal airway epithelium
Rhinitis
GSM475647
9.637234771 y
Male
Uncontrolled asthma UA4
Nasal airway epithelium
Uncontrolled asthma
GSM475648
13.92744695 y
Female
Healthy C9
Nasal airway epithelium
Healthy
GSM475649
10.19575633 y
Male
Healthy C10
Nasal airway epithelium
Healthy
GSM475650
10.94592745 y
Female
Rhinitis R8
Nasal airway epithelium
Rhinitis
GSM475651
13.08692676 y
Female
Rhinitis R13
Nasal airway epithelium
Rhinitis
GSM475652
13.66461328 y
Male
Rhinitis R9
Nasal airway epithelium
Rhinitis
GSM475653
10.16563997 y
Male
Rhinitis R14
Nasal airway epithelium
Rhinitis
GSM475654
8.930869268 y
Female
Uncontrolled asthma UA5
Nasal airway epithelium
Uncontrolled asthma
GSM475655
8.093086927 y
Male
Uncontrolled asthma UA6
Nasal airway epithelium
Uncontrolled asthma

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